TABLE 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| B. pseudomallei | ||
| 1026b | Clinical isolate; Gmr Smr Pxr Tps | 11 |
| DD503 | 1026b derivative; Δ(amrR-oprA) rpsL (Smr) AGs Tcs | 24 |
| ATL0378 | DD503 derivative; ΔBPSL0378 Gmr | This study |
| ATL1876 | DD503 derivative; ΔBPSL1876 Gmr | This study |
| ATL2025 | DD503 derivative; ΔBPSL2025 Gmr | This study |
| ATL2515 | DD503 derivative; ΔBPSL2515 Gmr | This study |
| ATL3050 | DD503 derivative; ΔBPSL3050 Gmr | This study |
| ATL3320 | DD503 derivative; ΔBPSL3320 Gmr | This study |
| ATS0067 | DD503 derivative; ΔBPSS0067 Gmr | This study |
| ATS0464 | DD503 derivative; ΔBPSS0464 Gmr | This study |
| ATS1388 | DD503 derivative; ΔBPSS1388 Gmr | This study |
| ATS1489 | DD503 derivative; ΔBPSS1489 Gmr | This study |
| ATS1530 | DD503 derivative; ΔBPSS1530 Gmr | This study |
| ATCPL2025 | ATL2025 carrying pCPL2025; Gmr Tmpr | This study |
| ATCPS0067 | ATS0067 carrying pCPS0067; Gmr Tmpr | This study |
| E. coli | ||
| TOP10 | General cloning and blue-white screening. | Invitrogen |
| SM10λpir | E. coli cloning strain | 30 |
| S17-1 | E. coli cloning strain | 30 |
| Plasmids | ||
| pCR2.1-TOPO | 3.9-kb TA cloning vector; pMB1 oriR Apr Kmr | Invitrogen |
| pGSV3-lux | Mobilizable suicide vector containing lux operon from pSC26; oriT Gmr | 25 |
| pUCP28T | Shuttle vectors derived from pUC18/19; Tmpr | 35 |
| PCPL2025 | (BamHI-XbaI)-pUCP28T::full BPSL2025; Tmpr | This study |
| PCPS0067 | (BamHI-XbaI)-pUCP28T::full BPSS0067; Tmpr | This study |