TABLE 2.
PCR primers used in this study
| Gene | Primer name | Nucleotide sequence, 5′ to 3′ | Purpose |
|---|---|---|---|
| BPSL0378 | L0378-for | CAAAGGCTCCCGATGAGCGA | Internal PCR |
| L0378-rev | AAATGTCGGCATCGAGCGCC | Internal PCR | |
| Up-L0378-for | GAAATTCGTGTCGAGCTGCG | Merodiploid confirmation | |
| BPSL1876 | L1876-for | GGTCGGCGTCTACCGGAT | Internal PCR |
| L1876-rev | TCGAGGAACGTGAACGCGGG | Internal PCR | |
| Up-L1876-for | CCCAACGGAGTCCGCTCGT | Merodiploid confirmation | |
| BPSL2025 | L2025-for | ACCGCGACCTGAAGCGGCT | Internal PCR |
| L2025-rev | GATCGACACGACCCGCCC | Internal PCR | |
| Up-L2025-for | TCGCGTTCCTGACGCTGCC | Merodiploid confirmation | |
| CPL2025-for | CCATAAGCGGAACGCCGCAG | Complementation of ΔBPSL2025 | |
| CPL2025-rev | TCGATACGTCGCCCATGCCC | Complementation of ΔBPSL2025 | |
| BPSL2515 | L2515-for | ATCAGGGCGAGGTTGAGGTT | Internal PCR |
| L2515-rev | ATGCGATGTCGGTGATGTG | Internal PCR | |
| Up-L2515-for | CGAACAGGTGCTCGCGTG | Merodiploid confirmation | |
| BPSL3050 | L3050-for | CCAACGAGGGCTACAACCGC | Internal PCR |
| L3050-rev | GCACGCTCGCCGACGTGA | Internal PCR | |
| Up-L3050-for | AATTCGGCAGGATCAGCG | Merodiploid confirmation | |
| BPSL3320 | L3320-for | GCCCTCGACGGGCAATTCGT | Internal PCR |
| L3320-rev | TTGCGACCATGCGACGCTGC | Internal PCR | |
| Up-L3320-for | GTCGACTTCGGCAACCAGCG | Merodiploid confirmation | |
| BPSS0067 | S0067-for | GATACCCGCCTTCGTCATCT | Internal PCR |
| S0067-rev | CAGCGTGCCGAAGTAGTTG | Internal PCR | |
| Up-S0067-for | GATACCCGCCTTCGTCATCT | Merodiploid confirmation | |
| CPS0067-for | CTCGATGTGCAACGCCGCC | Complementation of ΔBPSS0067 | |
| CPS0067-rev | CCATTGTCGTGCGCGTCAGG | Complementation of ΔBPSS0067 | |
| BPSS0464 | S0464-for | GGTCAGGCTTCTCGCTGAAG | Internal PCR |
| S0464-rev | TGATGAAGAGCGTCGCGAGC | Internal PCR | |
| Up-S0464-for | GGCTATCCGATGGCGTACTA | Merodiploid confirmation | |
| BPSS1388 | S1388-for | GCGAATCTCGCGAAAGCGGA | Internal PCR |
| S1388-rev | CCAGTCTCTTGGCGACCTTC | Internal PCR | |
| Up-S1388-for | TGTTCCGGCAACTCGAAACG | Merodiploid confirmation | |
| BPSS1489 | S1489-for | GCGACGGCAACCGATTCGCT | Internal PCR |
| S1489-rev | ACTGAATGCCGAGCAGCGCG | Internal PCR | |
| Up- S1489-for | GCGACGGCAACCGATTCGCT | Merodiploid confirmation | |
| BPSS1530 | S1530-for | AAGGAACTGAAGGCTCGGGC | Internal PCR |
| S1530-rev | TCCATTTCGGCTCGATGCCG | Internal PCR | |
| Up-S1530-for | AAGAACCAGATCGACGGCGC | Merodiploid confirmation | |
| luxC | luxC-rev | ACGAATGTATGTCCTGCGTCTTGAG | pGSV3-lux fusion and merodiploid confirmation |
| 16S rRNA | 16S rRNA-for | TGGCTAATACCCGGAGTGGA | RT-PCR |
| 16S rRNA-rev | CGGTTTGTCACCGGCAGTCT | RT-PCR | |
| 23S rRNA | 23S rRNA-for | GCCAGCTAAGGTCCCCAAAT | RT-PCR |
| 23S rRNA-rev | TGCAGGTCGGAACTTACCCG | RT-PCR |