TABLE 3.

List of 31 common genes of B. pseudomallei differentially expressed in all three organ infection models following i.p. inoculation

Gene	Annotation	Expression level for organ and preparation no.
		Liver				Lung				Spleen
		1		2		1		2		1		2
		Meana	SD	Meana	SD	Meana	SD	Meana	SD	Meana	SD	Meana	SD
BPSL0004	hupA, DNA-binding protein HU-alpha	−4.22	0.46	−5.86	0.66	−6.68	1.57	−4.15	0.90	−5.68	1.70	−5.04	0.67
BPSL0060	AraC family transcriptional regulator	2.49	0.65	3.36	0.36	2.62	0.48	1.07	0.24	2.13	0.24	2.81	0.84
BPSL0117	LysR family transcription regulatory protein	3.84	1.05	5.50	0.77	2.89	0.89	3.17	1.06	4.85	2.22	4.23	0.92
BPSL0317	Conserved hypothetical protein	3.80	0.36	3.87	0.61	4.42	0.52	2.53	0.41	3.22	1.54	4.66	0.74
BPSL0379	Putative short-chain dehydrogenase	−4.11	1.03	−3.93	0.54	−3.66	0.53	−2.91	0.96	−2.87	0.49	−2.18	0.82
BPSL0430	Putative thioesterase	5.14	0.54	4.41	0.33	3.08	0.53	2.25	1.11	1.93	0.38	3.78	0.48
BPSL0522	Putative membrane protein	−8.78	1.06	−5.96	0.59	−8.49	1.30	−4.12	0.45	−6.34	2.93	−5.52	0.87
BPSL0635	Conserved hypothetical protein	−5.19	1.05	−4.97	0.81	−3.11	0.83	−5.49	1.47	−3.25	0.34	−2.69	0.98
BPSL1058	Putative transposase	−9.40	1.03	−7.52	0.37	−6.93	0.64	−8.24	2.30	−7.34	0.42	−6.02	0.56
BPSL1119	Putative lipopolysaccharide biosynthesis-related protein	4.62	0.46	5.37	0.23	3.45	0.76	3.04	1.39	4.96	0.93	4.70	1.27
BPSL1356	ftsH, cell division protein	−3.11	0.34	−3.40	0.37	−2.85	0.20	−3.09	0.63	−2.73	0.12	−4.07	0.76
BPSL1392	Hypothetical protein	3.63	1.43	5.59	0.62	3.05	0.57	1.88	0.20	2.78	1.10	5.40	1.74
BPSL1592A	Conserved hypothetical protein	−5.57	0.25	−4.79	0.29	−4.43	0.75	−4.77	0.99	−4.00	0.45	−3.58	0.37
BPSL1836	cysA, sulfate transport ATP-binding protein	4.20	0.49	4.58	0.45	3.56	0.08	1.78	0.38	3.45	0.81	4.65	0.52
BPSL2040	Hypothetical protein	4.32	0.78	5.31	0.96	4.41	0.77	2.91	0.75	3.60	0.92	5.82	0.90
BPSL2082	Conserved hypothetical protein	4.34	1.72	6.26	0.95	4.72	0.87	2.40	0.48	5.18	0.73	5.74	1.57
BPSL2219	Putative phosphoserine aminotransferase	−5.40	1.15	−3.40	0.28	−3.28	0.70	−3.76	0.26	−3.89	1.30	−2.36	0.48
BPSL2557	cspA, cold shock-like protein	−6.15	2.79	−8.83	0.57	−7.20	1.88	−5.22	1.86	−5.62	1.12	−5.11	0.79
BPSL2881	Putative membrane protein	−6.99	0.26	−4.74	1.10	−4.39	1.15	−4.86	1.52	−3.44	0.83	−1.83	0.24
BPSL2902	Putative phosphoglycerate mutase	4.19	0.93	5.17	0.38	2.73	0.96	2.20	0.63	3.25	0.43	4.31	0.72
BPSL3027	murD, UDP-N-acetylmuramoylalanine-d-glutamate ligase	5.03	0.63	4.81	0.38	2.48	1.12	3.27	1.62	2.97	0.55	2.59	0.38
BPSL3050	trpE, anthranilate synthase component 1	3.94	1.93	5.42	0.33	2.72	0.81	2.24	1.02	2.49	0.70	3.55	1.26
BPSL3085	Conserved hypothetical protein	−3.46	0.29	−2.87	0.16	−2.56	0.60	−3.62	1.12	−2.60	0.67	−2.07	0.58
BPSL3253	Putative oxidoreductase	4.83	1.18	4.82	0.58	3.05	0.92	2.90	1.05	3.56	1.18	2.15	0.52
BPSS0515	Hypothetical protein	−6.63	0.6	−5.21	0.90	−5.35	2.04	−6.03	1.52	−4.40	1.62	−3.02	0.17
BPSS0965	Putative oxalate decarboxylase	3.81	0.48	5.93	0.20	3.47	0.21	2.25	1.04	3.93	0.15	3.96	1.35
BPSS1199	Hypothetical protein	−6.11	1.73	−7.50	0.63	−6.96	1.95	−4.64	2.01	−6.18	1.83	−4.96	0.1
BPSS1388	Putative membrane protein	5.56	1.53	7.86	1.05	5.40	1.33	2.34	0.65	4.59	0.44	5.14	1.36
BPSS1489	Putative exported protein	5.94	0.53	7.45	0.37	3.97	0.40	2.68	1.34	3.64	0.13	4.53	1.17
BPSS1933	Putative membrane protein	−6.43	0.58	−4.67	0.54	−4.05	0.64	−4.63	2.07	−4.51	0.38	−3.82	0.36
BPSS2229	Conserved hypothetical protein	2.90	1.08	3.45	0.27	2.40	1.04	2.22	0.43	2.02	0.44	3.25	1.11

^aMean value is mean log₂ ratio (in vivo/in vitro) calculated from two replicates.