FIG. 2.

Transepithelial PMN migration induced by wild-type S. flexneri. T84 polarized monolayers were infected with 2457T, BS771 (ΔospF), BS772 (ΔospC1), BS802(pBAD-OspF), BS803(pBAD-OspC1), and BS103, and then PMN migration was evaluated. HBSS, uninfected monolayers (buffer only). All strains were normalized to wild-type strain 2457T. Experiments were performed three times in triplicate. The data are means ± standard deviations (error bars) of triplicate samples and represent one of the three experiments performed in which similar results were obtained. Samples were compared using an unpaired t test, and statistically significant differences are indicated by an asterisk.