FIG. 3.

ERK phosphorylation in S. flexneri-infected cells. T84 polarized monolayers were infected with 2457T, BS771 (ΔospF), BS772 (ΔospC1), and BS103, and then ERK phosphorylation was evaluated 180 min postinvasion. HBSS, uninfected monolayers (buffer only). Lysates of monolayers were immunoblotted with monoclonal anti-P-ERK antibody (anti-P-ERK), which recognized the phosphorylated ERK1/2 proteins (p44 and p42), or anti-ERK1/2 antibody (anti-ERK1/2), which was a total protein control and demonstrated equal loading. The blot is representative of one experiment that was repeated three times.