TABLE 2.
Primers used in this study
| Purpose | Forward primer
|
Reverse primer
|
||
|---|---|---|---|---|
| Designation | Sequencea | Designation | Sequencea | |
| Amplify the cassette for deletion of ospF | OspFMutF | TATATCTATTTTATAGAGGACGTTTTCTATGCCCATAAAAAAGCCCTGTTGTGTAGGCTGGAGCTGCTTC | OspFMutR | GCTACAAGGTGGTGTAGCTGGCATCTTCTCTACTCTATCATCAAACGATACATATGAATATCCTCCTTAG |
| Amplify the cassette for deletion of ospC1 | OspC1MutF | ATTAAAACTGTTTTCATATAAGGTTCATTTTATGAATATATCAGAAACATGTGTAGGCTGGAGCTGCT | OspC1MutR | CTTTTGCTAAACGATATTCAATTTTGATTAAATATATTTATTGTCAGATGCATATGAATATCCTCCTTAG |
| Clone POspB into pDZ1 | OspBProF | AATAGGGGATCCTACCTGACGCTT | OspBProR | CTACATGGATCCGACTCCATATATTG |
| Clone ospF into pGEM-T | OspFF | GGATCCGGTACCATGCCCATAAAAAAGCCCTG | OspFR | AGATCTCTCTATCATCAAACGATAAAATGG |
| Clone ospC1 into pGEM-T | OspC1For | GGTACCATGAATATATCAGAAACACTG | OspC1Rev | AGATCTAATATATTTATTGTCAGATGTTG |
| Clone ipaH9.8 into pGEM-T | IpaH9.8F | GGTACCATGTTACCGATAAATAATAAC | IpaH9.8R | AGATCTTGAATGGTGCAGTTGTGAGCCG |
| Clone spvC into pGEM-T | SpvCF | GGTACCATGCCCATAAATAGGCCTAATC | SpvCR | AGATCTCTCTGTCATCAAACGATAAAACG |
a
Underlining identifies an engineered Acc65I, BglII, or EcoRI restriction site.