FIG. 6.

Immune mice are protected against subsequent conjunctival challenge. Levels of infection in both memory immune and control mice were measured by real-time PCR and quantitative parasite culture 10 days after conjunctival challenge with 10⁶ CMT. In panel a, the numbers of T. cruzi molecular equivalents per microgram of total DNA are shown for the nasal cavity (including the nasolacrimal duct), the lacrimal and harderian glands, the parotid and submandibular lymph nodes, and the spleen (*, P < 0.02 [Mann-Whitney U test]; n = 5). In panel b, parasite outgrowths from mononuclear cell preparations are shown for draining lymph nodes and spleen (**, P < 0.04 [Mann-Whitney U test]; n = 5). In both panels vertical bars represent the standard errors of the means.