TABLE 3.
Anti-B. melitensis LPS IgG subtype ELISA titers of mouse sera at 4 weeks after the first dose of vaccinea
| Group | Vaccine | Anti-B. melitensis LPS IgG subtype ELISA titer (mean ± SD)
|
|||
|---|---|---|---|---|---|
| IgG1 | IgG2a | IgG2b | IgG3 | ||
| 1 | B. melitensis LPS, s.c. | 579 ± 347 | 324 ± 162 | 263 ± 229 | 2,381 ± 622 |
| 2 | B. melitensis LPS, i.n. | 109 ± 82 | 92 ± 59 | 40 ± 23 | 960 ± 456 |
| 3 | B. melitensis LPS-GBOMP, s.c. | 6,031 ± 2,888 | 852 ± 137 | 677 ± 548 | 2,883 ± 1,024 |
| 4 | B. melitensis LPS-GBOMP, i.n. | 1,643 ± 742 | 138 ± 73 | 128 ± 110 | 915 ± 292 |
| 5 | Saline control, s.c. | <1 | <1 | <1 | <3 |
a
Mice were immunized either s.c. or i.n. Two doses of vaccine were given 4 weeks apart. Sera were collected from five mice of each group at 4 weeks after the first dose. The titers are expressed in OD units.