TABLE 6.

Log numbers of CFU in lungs, livers, and spleens of mice 8 weeks after challenge^a

Group	Vaccine	Log CFU (mean ± SD)
		Lungs	Livers	Spleens
1	B. melitensis LPS, s.c.	1.73 ± 0.87	1.79 ± 0.28	2.31 ± 1.08
2	B. melitensis LPS, i.n.	1.57 ± 0.69	1.91 ± 0.42	2.35 ± 0.63
3	B. melitensis LPS-GBOMP, s.c.	1.97 ± 0.63	1.55 ± 0.62	1.65 ± 0.86
4	B. melitensis LPS-GBOMP, i.n.	2.06 ± 0.80	1.69 ± 0.40	2.23 ± 1.27
5	Saline control	2.04 ± 0.67	1.86 ± 0.79	3.57 ± 0.81

^aMice were immunized either s.c. or i.n. Two doses of vaccine were given 4 weeks apart. Mice were challenged with 10⁴ CFU B. melitensis 16M 4 weeks after the second dose of vaccine. Lungs, livers, and spleens of mice were collected 8 weeks after challenge. Serial dilutions of organ homogenates were plated on brucella agar, and CFU were counted after 3 to 5 days of incubation. The limit of detection was 2 CFU per organ. The data are data from one representative experiment (n = 20).