FIG. 3.

Fraction P3 containing fatty acids inhibits GPI-induced TNF-α production. (A) Macrophages were incubated for 24 h in the presence of the six different fractions (P1 to P6) isolated from 2 × 10⁸ trophozoites. (B and C) Macrophages were incubated for 24 h in the presence of Pfα (α) alone or with fractions P1 to P6 (added 30 min before Pfα was added) (B) or with the six fractions extracted from noninfected erythrocytes (E1 to E6 added 30 min before Pfα was added) (C). Medium alone was used as a negative control (lanes M). The values are means ± standard deviations of triplicate samples in a representative experiment.