FIG. 2.

Real-time reverse transcription-PCR analysis of epithelial cell gene expression after infection with PAO1 and the rsmA mutant. The expression of (A) Jun, (B) FGF18, (C) KLF2, and (D) KLF6 following infection with PAO1 (black bars) and the rsmA mutant (white bars) was investigated at 0, 1.5, 3, and 6 h postinfection (with a starting MOI of 50:1). Gene expression is presented as the x-fold change relative to expression in uninfected cells and was normalized for cDNA content by using the housekeeping gene encoding HPRT. Data (means ± standard deviations [SD]) are representative of three independent biological experiments.