FIG. 3.

Real-time reverse transcription-PCR analysis of KLF2 and KLF6 expression in response to live bacteria, bacterially conditioned cell-free supernatant, heat-killed bacteria, and LPS. The expression of (A) KLF2 and (B) KLF6 following 3 h of exposure to live bacteria or selected bacterial preparations (with a starting MOI of 50:1 for live bacteria and equivalent MOIs for bacterial preparations) is shown. LPS was used at a final concentration of 10 μg/ml. Gene expression is presented as the x-fold change relative to expression in uninfected cells and was normalized for cDNA content by using the housekeeping gene encoding HPRT. Data (means ± SD) are representative of three independent biological experiments.