FIG. 5.

Influence of ExoS, ExoT, and ExoY on gene expression of KLF2 and KLF6, using real-time reverse transcription-PCR. Gene expression of (A) KLF2 and (B) KLF6 at 3 h postinfection (with a starting MOI of 50:1) is presented as the x-fold change relative to expression in uninfected cells and was normalized for cDNA content by using the housekeeping gene encoding HPRT. Data (means ± SD) are representative of three independent biological experiments. The single, double, and triple mutants of exoS, exoT, and exoY are derivatives of a parental strain of P. aeruginosa PAO1 (RP289), as described previously (59).