FIG. 2.

Effects of inhibitors of various signaling pathways on the 3OC12-HSL-induced chemotaxis of PMN. PMN were preincubated with increasing doses (shown on the x axis) of Gefinitib (A) or pertussis toxin (B) for 10 min. After washing, chemotaxis toward 3OC12-HSL (100 μM) (•) was measured; the open circles show random migration, and the asterisks indicate that the means differed from the mean for the sample without Gefinitib (P < 0.002). For comparison chemotaxis toward IL-8 was measured (8 ng/ml) (right panels) (the results for only the highest concentration of the inhibitor are shown). The experiments were carried out with cells from different donors, which explains the differences in the chemotactic activities toward 3OC12-HSL and IL-8.