FIG. 4.

Uptake of ¹⁴C-labeled 3OC12-HSL by PMN. (A) PMN from a healthy donor were incubated with increasing concentrations of [¹⁴C]3OC12-HSL at 4°C for 20 min, and then the radioactivity associated with the cells was estimated (note that the x axis is linear for concentrations between 0 and 1 μM but logarithmic [log₁₀] for concentrations from 1 to 100 μM). (B) Binding of [¹⁴C]3OC12-HSL (0.1 μM) to PMN could be inhibited by an excess of unlabeled 3OC12-HSL.