FIG.3.

Chemokine mRNA changes in the lungs of susceptible (C3H/HeN and BALB/c) and resistant (C57BL/6N) mice as determined by using arrays. Total RNA was isolated from lungs of individual C57BL/6N (A), BALB/c (B), and C3H/HeN (C) mice, and pooled RNA from each experiment (n = 3 per experiment for each time point [two experiments]) was used to probe membrane-based cDNA chemokine microarrays at 3, 14, and 18 days after infection. The intensities of the spots were normalized using the intensity of the reactivity to the housekeeping gene, β-actin, and the results from infected mice were compared to those obtained from the RNA collected from the lungs of control mice inoculated with mycoplasma broth medium. Asterisks indicate cytokines where changes in mRNA expression as determined by spot intensity were at least an average of 2-fold, with no individual value being ≤1.5-fold.