TABLE 1.
Strains and plasmids used in the study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| A. hydrophila strains | ||
| SSU | CDC, Atlanta, Ga.a | |
| SSU-R | Rifampin-resistant (Rifr) strain of A. hydrophila SSU | Laboratory stock |
| Control | A. hydrophila SSU-R harboring pBAD, Rifr Apr | 6 |
| Dam-overproducing strain | A. hydrophila SSU-R harboring pBAD-damAhSSU, Rifr Apr | 6 |
| D/A Dam mutant | A. hydrophila SSU-R harboring pBAD-damD/A, Rifr Apr | This study |
| Y/A Dam mutant | A. hydrophila SSU-R harboring pBAD-damY/A, Rifr Apr | This study |
| MgidA | gidA isogenic mutant of A. hydrophila SSU-R, Rifr Kmr | 31 |
| MgidA/pBAD | gidA isogenic mutant of A. hydrophila SSU-R harboring pBAD plasmid, Rifr Kmr Apr | This study |
| MgidA/pBAD-damAhSSU | gidA isogenic mutant of A. hydrophila SSU-R harboring pBAD-damAhSSU plasmid, Rifr Kmr Apr | This study |
| E. coli strains | ||
| ES1301 mutS | lacZ53 mutS201::Tn5 thyA36 rha-5 metB1 deoC | Promega |
| JM109 | endA1 recA1 gyrA96 thi hsdR17 relA1 supE44 λ− Δ(lac-proAB) [F′ traD36 proA+B+laclZΔM15] | Promega |
| GM33 | F−dam-3 sup-85 (Am) | 25 |
| C. violaceum CV026 | Mini-Tn5 mutant of ATCC 31532 | 26 |
| Plasmids | ||
| pALTER-1 | Vector for the damAhSSU gene cloning, Tcr | Promega |
| pALTER-1/damAhSSU | dam gene of A. hydrophila cloned at the EcoRI/BamHI sites of pALTER-1 vector for mutagenesis, Tcr | This study |
| pALTER-1/damD/A | pALTER-1 vector containing damD/A mutation, Apr | This study |
| pALTER-1/damY/A | pALTER-1 vector containing damY/A mutation, Apr | This study |
| pBAD/Thio-E | araBAD promoter expression vector, Apr | Invitrogen |
| pBAD | Control vector, Apr | 6 |
| pBAD/damAhSSU | dam gene of A. hydrophila cloned into pBAD/Thio-E, Apr | 6 |
| pBAD/damD/A | pBAD vector carrying damD/A mutation, Apr | This study |
| pBAD/damY/A | pBAD vector carrying damY/A mutation, Apr | This study |
| pBAD/damD71A | pBAD vector carrying damD71A mutation, Apr | This study |
| pBAD/damE76A | pBAD vector carrying damE76A mutation, Apr | This study |
a
CDC, Centers for Disease Control and Prevention.