TABLE 4.
L. interrogans genes of known or predicted function which were up-regulated at physiological temperatures compared with environmental temperatures in three sets of biological replicates
| Function and ORFa | Mean fold up-regulation | Gene | COG categoryb | Description of gene product |
|---|---|---|---|---|
| Information storage and processing | ||||
| LA1970 | 1.5 | J | poly(A) polymerase family of proteins | |
| LA0876 | 1.5 | rpoE | K | RNA polymerase sigma subunit |
| LA1282 | 2 | K | Transcriptional regulator, AcrR family | |
| LA1816 | 1.5 | K | Transcriptional regulator | |
| LA2728 | 1.9 | K | Transcriptional regulator | |
| LA3652 | 2 | K | RNA polymerase sigma subunit | |
| LA4298 | 1.8 | K | Transcriptional regulator, MarR family | |
| LB104 | 1.7 | K | AcrR family transcriptional regulator | |
| LA0984 | 1.9 | L | ATP-dependent DNA ligase | |
| LA1456 | 2 | radC | L | DNA repair protein |
| Cellular processes and signaling | ||||
| LA0145 | 1.7 | D | ATPase of the PP-loop superfamily | |
| LA1837 | 1.7 | D | ParA-related protein | |
| LA1124 | 1.5 | lnt | M | Apolipoprotein N-acyltransferase |
| LA1283 | 1.9 | M | Conserved hypothetical protein | |
| LA1284 | 1.8 | lolE | M | Lipoprotein releasing system, LolE permease component |
| LA1504 | 2 | M | Metallopeptidase | |
| LA3489 | 1.6 | flgC | N | Endoflagellar proximal basal body rod protein |
| LA1231 | 2.1 | O | Heat shock protein HtpG | |
| LA1563 | 2.4 | O | Small heat shock protein (molecular chaperone) | |
| LA1564 | 1.7 | O | Small heat shock protein (molecular chaperone) | |
| LA1879 | 3.5 | clpA | O | Endopeptidase Clp, ATP-dependent proteolytic subunit |
| LA4299 | 1.8 | btuE | O | Glutathione peroxidase |
| LB174 | 1.6 | htpX | O | Zn-dependent protease with chaperone function |
| LA0816 | 1.7 | T | Receiver component of a two-component response regulator | |
| LA1212 | 1.9 | uspA | T | Universal stress protein UspA |
| LA1743 | 3.1 | T | Chemotaxis protein, methyltransferase | |
| LA1744 | 1.8 | T | Chemotaxis protein, methylesterase | |
| LA1746 | 1.7 | T | Sensor protein of a two-component response regulator, regulator protein of a two-component response regulator | |
| LA1860 | 1.6 | T | Sensor protein of a two-component response regulator | |
| LA2117 | 1.6 | T | Anti-sigma factor antagonist | |
| LA2223 | 1.7 | T | Sensor histidine kinase of a two-component response regulator | |
| LA3657 | 1.6 | T | Receiver component of a response regulator | |
| LA3858 | 1.6 | T | Sensor histidine kinase of a two-component complex, part; response regulator of a two-component complex, part | |
| LA0802 | 2 | U | Pilus assembly protein | |
| LA3754 | 1.5 | lepB | U | Signal peptidase I |
| LA0150 | 2.1 | V | Permease of an ABC transporter complex, ATP-binding protein of an ABC transporter complex | |
| LA1285 | 1.9 | V | Lipoprotein releasing system, LolD ATPase component | |
| Metabolism | ||||
| LA2453 | 1.6 | C | 4Fe-4S binding protein | |
| LA4164 | 1.5 | E | Zinc metalloprotease (elastase) | |
| LA1372 | 1.5 | G | Permease | |
| LA1457 | 1.6 | G | Membrane protein of an ABC transporter complex | |
| LA2127 | 1.6 | G | Epimerase | |
| LB102 | 2.3 | G | Sugar phosphatase | |
| LB105 | 1.5 | ubiG | H | Transcriptional regulator, ArsR family |
| LA0502 | 1.5 | desA | I | Fatty acid desaturase |
| LA2958 | 2 | I | Hydrolase, acyltransferase | |
| LA3928 | 1.5 | caiA | I | Acyl-CoA dehydrogenase |
| LA0593 | 2.4 | copZ | P | Copper chaperone |
| LA0594 | 3.1 | P | Cation transport ATPase, possibly copper | |
| LA1859 | 2.2 | katE | P | Catalase |
| LA3110 | 1.6 | kdpC | P | Potassium-transporting ATPase C chain |
| LA3242 | 2 | P | TonB-dependent receptor | |
| LA3972 | 1.5 | trkA | P | Potassium uptake system, NAD-binding component |
| LA3712 | 1.9 | Q | Permease component of an ABC transporter complex |
a
Ninety-three ORFs coding for hypothetical proteins (those grouped into COG R, S, or − or which are conserved or unique hypothetical proteins) were omitted from this table. For a complete listing, see Table S2 in the supplemental material.
b
COG categories are as described for Fig. 3.