FIG. 4.

p38 MAP kinase mediates the production of IFN-γ in B. burgdorferi-specific effector Th1 cells in response to TCR signals. CD4⁺ T cells were purified from C3H mice infected for 2 weeks, and they were restimulated with plate-bound anti-CD3 (αCD3) in the absence or presence of 5 μM SB203580 (SB). IFN-γ levels in the restimulation supernatants were determined after 40 h by capture ELISA. The results are representative of the results of three independent experiments. The asterisk indicates that the P value is <0.01, as determined by Student's t test.