FIG. 2.

IFN-γ signaling pathway and gene regulation by IRF-1. (A) IFN-γ pathway genes induced (red) in 44 PBMC samples from 22 paired infected and uninfected subjects. (B) Identification of statistically significant (P < 0.001) sets of genes (12 genes identified) whose promoter region binding sites are homologous to the known IRF-1 DNA-binding site using CLOVER with the default cutoff significance set at a P value of 0.01. Promoter regions were aligned, and the consensus IRF-1 binding site was determined and depicted using a sequence logo generator. (C) Correlation plot of the normalized expression value of IRF-1 from 22 uninfected and 22 malaria-infected samples with the mean gene expression level of 12 genes. (D) Heat map showing individual normalized gene expression of IRF-1 with each of the genes identified by clover as having an overrepresented IRF-1 binding site in the gene promoter.