FIG. 7.

Synergism of purified gingipains in induction of CAM cleavage. Fifteen micrograms of total protein from BCAEC treated for 6 h (N-cadherin), 24 h (VE-cadherin), or 12 h (integrin β1), in the presence of 5 mM l-cysteine, with purified HRgpA (8 μg/ml), Kgp (3 μg/ml), or RgpB (5.2 μg/ml) (all equivalent to 113 U of Rgp activity/ml media or 12.4 U of Kgp activity/ml media) were separated by SDS-PAGE and immunoblotted with antibodies to N-cadherin, VE-cadherin, and integrin β1. Arrows indicate cleavage products, whereas lines indicate intact proteins. Un, untreated; +, present.