TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
| B. pertussis | ||
| Tohama | Wild-type strain | NVIa |
| Tohama Bvg+ phase lock | Bvg+ phase-locked mutant of B. pertussis Tohama strain | This study |
| E. coli | ||
| TOP10F′ | F′[lacIq Tn10 (Tetr)] mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG | Invitrogen |
| DH5α | F− Δ(lacZYA-algF)U169 thi-1 hsdR17 gyrA96 recA1 endA1 supE44 relA1 phoA φ80 ΔlacZΔM15 | 22 |
| SM10λpir | thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu λ pir R6K Kanr | NVI |
| N. meningitidis | ||
| H44/67 | Wild-type strain | NVI |
| H44/67-lpxA | LPS-deficient derivative of H44/76 | 53 |
| Plasmids | ||
| pCRII-TOPO | E. coli cloning vector; Ampr Kanr | Invitrogen |
| pET-11a | E. coli high-copy expression vector, Ampr, T7 promoter | Novagen |
| pMMB67EH | Broad-host-range expression vector | 15 |
| pPagL(Pa) | pET-11a derivative harboring P. aeruginosa pagL | 17 |
| pPagL(Bb) | pET-11a derivative harboring B. bronchiseptica pagL | 17 |
| pPagL(Pa)(S151A) | pPagL(Pa) encoding PagL(Pa) with S151A substitution | 17 |
| pPagP(Bp) | pET-11a derivative harboring B. pertussis pagP | This study |
| pMMB67EH-PagL(Pa) | pMMB67-EH derivative harboring P. aeruginosa pagL | This study |
| pMMB67EH-PagL(Bb) | pMMB67-EH derivative harboring B. bronchiseptica pagL | This study |
| pMMB67EH-PagL(Pa)(S151A) | pMMB67EH-PagL(Pa) encoding PagL(Pa) with S151A substitution | This study |
| pMMB67EH-PagP(Bp) | pMMB67-EH derivative harboring B. pertussis pagP | This study |
| pMMB67EH-PagP(Ec) | pMMB67-EH derivative harboring E. coli pagP | 55 |
| pEN11-PagL | pEN11 derivative harboring B. bronchiseptica pagL | 8 |
| pJM503 | Suicide vector harboring bvgS region with a G2527A substitution | 35 |
a
NVI, Netherlands Vaccine Institute, Bithoven, The Netherlands.