TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristicsb | Source or reference |
|---|---|---|
| Y. pestisa | ||
| KIM5-3001 | Smr pCD1 pPCP1 pMT1 | 30 |
| KIM5-3001.6 (ΔyopN) | Smr pCD1 yopN pPCP1 pMT1 | 46 |
| KIM5-3001.P39 (ΔyopE) | Smr pCD1 sycE yopE::Km pPCP1 pMT1 | 13 |
| KIM5-3001.P64 (ΔyopEB) | Smr pCD1 sycE yopE::Km yopB pPCP1 pMT1 | 58 |
| KIM5-3001.P55 (ΔyopN ΔtyeA) | Smr pCD1 yopN tyeA pPCP1 pMT1 | 18 |
| KIM5-3001.P62 (ΔyopN) | Smr pCD1 sycE yopE::Km yopN pPCP1 pMT1 | 19 |
| KIM5-3001.P68 (ΔyopN ΔsycN) | Smr pCD1 sycE yopE::Km yopN sycN pPCP1 pMT1 | 19 |
| KIM5-3001.P69 (ΔyopN ΔlcrG) | Smr pCD1 sycE yopE::Km yopN lcrG pPCP1 pMT1 | 19 |
| KIM5-3001.P70 (ΔyopN ΔyscB) | Smr pCD1 yopN yscB pPCP1 pMT1 | 19 |
| KIM5-3001.P48 (yop polymutant) | Smr pCD1 sycE yopE::Km yopJ yopT yopM yopH ypkA lcrQ pPCP1 pMT1 | 13 |
| KIM5-3001.P49 (yop polymutant yopB) | Smr pCD1 sycE yopE::Km yopJ yopT yopM yopH ypkA lcrQ yopB pPCP1 pMT1 | 13 |
| KIM5-3001.P72 (yop polymutant yopB yopN) | Smr pCD1 sycE yopE::Km yopJ yopT yopM yopH ypkA lcrQ yopB yopN pPCP1 pMT1 | 13 |
| KIM8-3002 (parent) | Smr pCD1 pMT1 | 64 |
| KIM8-3002.P39 (ΔyopE) | Smr pCD1 sycE yopE::Km pMT1 | 13 |
| E. coli DH5α | F− Φ80 dlacZ ΔM15 Δ(lacZYA argF)U169 endAl recAl hsdR17 deoR supE44 thi-1 gyrA96 relA1 | 7 |
| H. pylori P12ΔcagA | ΔcagA::Km | 40 |
| Plasmidsc | ||
| pBluescriptKS− | Cloning vector; Apr | Stratagene |
| pBAD30 | Expression vector; Apr | 24 |
| pYopE | 1.53-kb XhoI-BamHI fragment encoding YopE and SycE inserted into XhoI- and BamHI-digested pBluescriptKS− | 13 |
| pYopE129-ELK | 1.19-kb PstI-BamHI fragment encoding YopE1-129-ELK and SycE in pBluescriptKS− | 13 |
| pYopB2 | yopB complementation plasmid; Cmr | 13 |
| pIP9 | E. coli-H. pylori shuttle vector encoding GSK-CagA | 25 |
| pWS252 | E. coli-H. pylori shuttle vector encoding GSK-CagA1-1194 | 25 |
a
All Y. pestis strains are avirulent because of deletion of the pgm locus (59).
b
Native plasmids of Y. pestis include pCD1 (43); pPCP1 (54), encoding the outer membrane plasminogen activator (Pla) protease that has been shown to degrade exported Yops; and pMT1, encoding the capsular protein (47).
c
GSK tag and ELK tag expression plasmids are described in Materials and Methods.