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Binding of rDBL3γ to CSA. Binding of rDBL3γ to Bio-CSA, Bio-CSB, Bio-CSC, and Bio-HA immobilized on streptavidin-coated microwells was detected using a mouse monoclonal antibody against penta-His. Recombinant PvRII, the binding domain of PvDBP, and recombinant gC1qR/HABP1, which binds HA, were used as control ligands. rDBL3γ binds CSA but not CSB, CSC, or HA. gC1qR/HABP1 binds HA but not CSA, CSB, or CSC. PvRII does not bind any of the receptors tested. OD, optical density.
