FIG. 4.

Recognition of surfaces of P. falciparum IEs by anti-rDBL3γ mouse sera using L-IFA. Mouse sera raised against rDBL3γ of FCR3 var1CSA react with the IE surface of P. falciparum laboratory strain FCR3CSA and that of placental isolate 193CSA, which bind CSA, but not with that of P. falciparum laboratory strain FCR3CD36 or that of peripheral field isolate JDP8, which do not bind CSA. Parasites were stained with DNA-intercalating dye DAPI (blue) to identify IEs and with anti-rDBL3γ mouse sera followed by Alexafluor 488-conjugated chicken anti-mouse IgG (green).