TABLE 2.
Inhibition of adhesion of P. falciparum isolates to SBEC C2 cells under static conditions and to placental cryosections under flow conditions by anti-rDBL3γ mouse sera
| Parasite strain | % Inhibition by anti-rDBL3 γ mouse sera (avg ± SD) of IE binding to:
|
|
|---|---|---|
| SBEC C2 (static)a | Placenta (flow)b | |
| FCR3CSA | 86 ± 1 | 76 ± 5 |
| BC1-1CSA | 90 ± 3 | 82 ± 5 |
| 24-CSA | 86 ± 3 | 88 ± 2 |
| 42DJ-CSA | 86 ± 4 | 79 ± 9 |
| 42-CSA | 90 ± 5 | 93 ± 3 |
| 193-CSA | 91 ± 1 | 93 ± 9 |
| 938-CSA | 88 ± 3 | 90 ± 4 |
| 939-CSA | 89 ± 3 | 83 ± 4 |
Anti-rDBL3γ mouse sera (1:20 dilution) were tested for inhibition of binding of IEs to SBEC C2 cells in static binding assays. Binding in the presence of preimmune mouse sera (1:20 dilution) was used as a control to determine inhibition efficiencies of anti-rDBL3γ sera. The number of IEs bound to SBEC C2 cells was scored in five random fields in each well by Giemsa staining. Results represent inhibition efficiencies (average ± standard deviation) determined from three independent experiments. Each assay was performed in duplicate wells in each experiment. The number of IEs bound to SBEC C2 cells in control wells was in the range of 90 to 125 bound IEs per field.
Anti-rDBL3γ pooled mouse sera were tested at a dilution of 1:20 for the inhibition of adhesion of IEs to placental cryosections under flow conditions. Binding in the presence of preimmune mouse sera (1:20 dilution) was used as a reference to determine the inhibition efficiencies of anti-rDBL3γ sera. Results represent inhibition efficiencies (average ± standard deviation) determined from two independent experiments. Assays were performed with two different placental cryosections, each of which was mounted in duplicate flow cells for each experiment. The number of bound IEs was scored in 10 fields per flow cell in both experiments. The number of bound IEs in the presence of preimmune serum was used as a reference. The number of bound IEs in control wells was in the range of 213 to 328 per field.