TABLE 4.
Inhibition of binding of CSA to DBL3γ domain of FCR3 var1CSA and DBLX domains of 3D7 var2CSA with mouse sera raised against rDBL3γ
| Construct | Transfection efficiency (%) | Efficiency of Bio-CSA binding (%) after preincubation witha:
|
||||
|---|---|---|---|---|---|---|
| Preimmune sera | Anti- rDBL3γ sera | CSA | CSB | CSC | ||
| pRE4-DBL3γ | 79 ± 3 | 81 ± 3 | 0 | 0 | 76 ± 12 | 72 ± 6 |
| pRE4-DBL1X | 68 ± 5 | 0 | 0 | 0 | 0 | 0 |
| pRE4-DBL2X | 61 ± 6 | 57 ± 4 | 55 ± 6 | 0 | 54 ± 6 | 64 ± 5 |
| pRE4-DBL3X | 66 ± 6 | 62 ± 3 | 0 | 0 | 68 ± 9 | 63 ± 8 |
| pRE4-DBL4ɛ | 58 ± 4 | 0 | 0 | 0 | 0 | 0 |
| pRE4-DBL5ɛ | 60 ± 6 | 0 | 0 | 0 | 0 | 0 |
| pRE4-DBL6ɛ | 51 ± 5 | 0 | 0 | 0 | 0 | 0 |
a
Transfected 293T cells were preincubated with preimmune mouse sera (1:20 dilution), anti-rDBL3γ mouse sera (1:20 dilution), CSA (200 μg ml−1), CSB (200 μg ml−1), and CSC (200 μg ml−1) prior to incubation with Bio-CSA. The efficiency of Bio-CSA binding was determined as described for Table 3. Results reported are average± standard deviation of three independent experiments. Where the binding efficiency is reported as zero, no Alexafluor 488-stained cells were seen in the entire well.