FIG. 3.

Identification of a fibronectin-binding protein of Vibrio vulnificus. A crude lysate of wild-type V. vulnificus was added to a multiwell plate coated with 50 μg of fibronectin and incubated for 3 h. After being washed with PBS, the proteins bound to the fibronectin-coated surface were eluted with 1% sodium dodecyl sulfate and concentrated fivefold with Centricon as described in Materials and Methods. Upon separation by 10% SDS-PAGE and transfer to a PVDF membrane, the eluted proteins were visualized by Ponceau. A protein of 35 kDa was excised and subjected to N-terminal amino acid sequencing.