TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | supE44 ΔlacU169 (φ80 lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | Laboratory collection |
| SM10λpir | thi thr leu tonA lacY supE recA::RP4-2-Tc::Muλpir; OriT of RP4 Kmr; conjugational donor | 20 |
| JM109 | recA1 supE44 endA1 hsdR17 gyrA96 relA1 thi Δ(lac-proAB) [F′ traD36 proAB′ lacIqZΔM15] | 44 |
| V. vulnificus | ||
| MO6-24/O | Clinical isolate | 41 |
| DK1 | ΔompU mutant of MO6-24/O | This study |
| Plasmids | ||
| pGEMT-Easy | Vector used for cloning of PCR product | Promega |
| pDK1 | pGEMT-Easy ompU+ | 28 |
| pTrcHisB | Expression vector for a histidine-tagged protein | Invitrogen |
| pTrcompU | pTrcHisB ompU′ | 28 |
| pBluescript SK(−) | OriR6K OriT AprrpsL | Stratagene |
| pBS-DK1 | pBluescript SK(−) ompU+ | This study |
| pBS-dU | pBS-DK1 ΔompU | This study |
| pDM4 | Suicide vector; OriR6K Cmr | 21 |
| pDM-dU | pDM4 ΔompU | This study |
| pCOS5 | OriV OriT Apr Cmrcos | 3 |
| pJH0311 | pCOS5, multicloning sites; Apr | S. H. Choi |
| pSM1 | pJH0311 ompU+ | This study |