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. 2000 Mar 14;97(6):2462–2467. doi: 10.1073/pnas.97.6.2462

Figure 1.

Figure 1

Rsk-2 is absent in 3T3 cell lines from KO embryos. (A) PCR genotyping to identify male embryos (Top) and the presence of the neomycin-resistance gene in the targeted Rsk-2 allele (Middle). Western blot analyses using Rsk-2-specific antibody on cell extracts from primary mouse embryonic fibroblasts (Bottom). (B) Western blot analysis on extracts from immortalized 3T3 cells of control embryo no. 7 (WT) and KO embryo no. 6 (KO) with Rsk-1-specific (Upper) and Rsk-2-specific antibodies (Lower).