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. 2000 Mar 7;97(6):2468–2473. doi: 10.1073/pnas.040476297

Figure 5.

Figure 5

Squelching of the suppressor protein by competing with the CCTTG repeat. (A) A luciferase reporter construct containing the wild-type γ-globin promoter (γ-luc) or a luciferase reporter construct containing the γ-globin promoter with the 31-bp suppressor binding region deleted (γΔ-luc) was cotransfected with either empty pBlueScript SK(+) (Stratagene) vector (open bar), a plasmid containing 15 copies of CCTTG repeat (solid bar), or a plasmid containing 15 copies of CCGAG mutated repeat (striped bar). Luciferase assays were performed 48 hr after transfection into MEL (Left) or K562 cells (Right). (B) A luciferase reporter construct containing the enhancerless SV40 promoter (SV40-luc) or a reporter construct containing the SV40 promoter with four copies of the CCTTG repeats inserted upstream [SV40(4R)-luc] or two copies of the CCTTG repeats [SV40(2R)-luc] was cotransfected with the competitor plasmids into K562 cells. Luciferase assays were performed as described in A. Results with MEL cells were very similar (data not shown).