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Analysis of Sult1C1 and its binding to PAP by DSF and DSLS. (A and B) Thermostability of Sult1C1 in the presence of 0 (●), 0.08 (○), 0.35 (▾), 1.4 (▵), 5.6 (■), 11.2 (□), 22.5 (⧫), and 45 (◇) mM PAP measured by DSF (A) and DSLS (B). (C and D) The increases in the thermal stability, ΔTm and ΔTagg, as a function of the concentration of PAP, measured by DSF and DSLS, are shown in C and D, respectively.
