Fig. 1.

Diagram of the Slc25a19 knockout strategy. A 2.5-kb EcoRI (E)/BamHI (B) fragment (5′) and a 3.9-kb SpeI (S)/EcoRI (3′) fragment were cloned into pPNT-far. The construct is missing exons 4–6. Transfected ES cell clones were digested with either BamHI (B) (3′ and neo probes) or NheI (N) (5′ probe) to test for homologous recombination. Thick lines represent mouse sequence, thin lines represent vector sequence, and the arrow represents the Neo^R cassette. The stippled boxes represent the Southern blot analysis probes. Black numbered boxes are exons (the translation start site is in exon 2).