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. 2006 Oct 11;103(43):15927–15932. doi: 10.1073/pnas.0607661103

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© 2006 by The National Academy of Sciences of the USA

Freely available online through the PNAS open access option.

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Fig. 4. — AKG levels in amniotic fluid from 25 embryos at E10.5. AKG was measured by using tandem MS and an internal standard. Five embryos were Slc25a19^+/+ (triangles), 13 embryos were Slc25a19^+/− (squares), and 7 embryos were Slc25a19^−/− (circles). The samples represented by each color were measured during the same experiment. The mean values were 6.17 ± 0.72 μmol/liter for the unaffected and 30.8 ± 9.91 μmol/liter for the mutants (P = 0.0155 Mann–Whitney). The data also were dichotomized by using the mean of the entire data set (13.06) and tested with Fisher's exact test (P = 0.0004). The t test could not be used because the data were not normally distributed.