FIG. 2.

Immunoprecipitation of free LPS. SDS-PAGE gel showing the amounts of LPS that were immunoprecipitated by VHHs immobilized to Talon beads. (A) Three LPS derivatives from N. meningitidis H44/76 (L3, L8, and galE) were efficiently precipitated by all four anti-LPS VHHs. (B) LPS from E. coli B4:O111, Re LPS from Salmonella serovar Typhimurium, and LPS from B. pertussis were precipitated with the VHHs 5G, 7H, and 12H but not with VHH 7F. In both panels, the lanes marked std represent input LPS, i.e., the maximal amount of LPS that could be immunoprecipitated. The lanes marked C show LPS that was precipitated with an irrelevant VHH. Lane 1 contains the molecular mass standard in kDa (indicated at the left). In panel B, the larger part of the gel is shown to include the O antigen containing high-molecular-mass LPS bands of E. coli B4:O111.