FIG. 6.

Sequestration of the L3 LPS from an aqueous solution by VHHs. Graph showing the amounts of luminescence (y axis) produced by an LPS solution (2 μg/ml) before (input) and after incubation with the indicated anti-LPS VHHs, which were immobilized through the histidine tag to Talon beads. As controls, empty Talon beads (BD) and beads loaded with an irrelevant VHH (C) were used. Both equivalent amounts (−1) and 10-fold the amount (−10) of input were used in the assay. The inhibition of luminescence by the anti-CD14 MAb 60bca is also indicated. The experiment was repeated three times, and error bars are indicated.