FIG. 2.

FPLC gel filtration (size exclusion chromatography) of unstimulated pooled whole saliva. Unstimulated whole saliva was separated by gel filtration chromatography (FPLC) on a Superose 12 column (Pharmacia), with the elution of proteins monitored at 280 nm. The seven fractions were tested for HIV-1 inhibitory activity using the 3-day microtiter assay (as shown by the open bars), with levels based on percentages of reduction in HIV-1 p24 antigen (p24Ag) production (pg/ml). Standard marker proteins are denoted at the top, and the positions of recombinant MG2, sheep breast milk lactoferrin, and recombinant SLPI are shown at the bottom.