FIG. 3.

FPLC gel filtration chromatography of pooled breast milk. Pooled breast milk was separated by FPLC gel filtration chromatography on a Superose 12 column (Pharmacia), with the elution of proteins monitored at 280 nm, and then divided into nine main fractions. Each fraction was tested for HIV-1 inhibitory activity using the 3-day microtiter assay (as shown by the open bars), with levels based on percentages of reduction in HIV-1 p24 antigen (p24Ag) production (pg/ml). Standard marker proteins are denoted at the top, and the positions of recombinant MG2, sheep breast milk lactoferrin, and recombinant SLPI are shown at the bottom.