FIG. 7.

Variability of immunoblots profile. Every lane was loaded with 10 μg of M. paratuberculosis CF proteins from organism grown in WR medium. SDS-PAGE was done with 10 to 20% gradient gels. After protein transfer to nitrocellulose, serum was applied, incubated, and stained with rabbit anti-bovine IgG HRP conjugate and diaminobenzidine substrate. The reactivity of sera from 31 M. paratuberculosis-infected cattle, after absorption with M. phlei CE proteins, is shown in panel A. The reactivity of sera from six noninfected cattle is shown in panel B, both preabsorption and postabsorption with M. phlei or M. paratuberculosis. Absorption was done by using 1,500 of CE protein/ml.