FIG. 3.

Alignment and expression of AgBoi1/2p. (A) Alignment of A. gossypii AgBoi1/2p with S. cerevisiae Boi1p and Boi2p. Individual domains are marked with boxes, and the identity between corresponding domains is indicated (generated with the application “needle” from “EMBOSS” [43]). The sequences of the partial proteins AgBoi1/2pΔN and AgBoi1/2pΔC are indicated below the AgBoi1/2p sequence, with the thin lines marking the respective deletions. (B) Western blot of wild-type AgBoi1/2p and truncated proteins. Proteins from 16-h cultures were extracted, separated on an SDS-polyacrylamide gel electrophoresis gel, transferred to a membrane, and hybridized with an anti-GFP antibody. Molecular masses from a standard protein marker are indicated in kilodaltons. The molecular mass of the GFP epitope tag with a linker is 28 kDa. A degradation product was detected for AgBoi1/2pΔC at ∼65 kDa.