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. 2006 Sep 1;5(10):1635–1647. doi: 10.1128/EC.00210-06

FIG. 7.

FIG. 7.

AgBoi1/2p-AgRho3p-AgBni1p two-hybrid interactions. (A) AgBoi1/2p-AgRho3p interaction. In the left column, the (truncated) AgBoi1/2p fused to the activation domain and the activation domain alone are listed, and in the top row the AgRho3p fused to the binding domain and the binding domain alone are listed. (B) AgRho3p-AgBni1p interaction. In the left column the AgBni1 protein fused to the activation domain (47) and the activation domain alone are listed. In the top row, the AgRho3p fused to the binding domain and the binding domain alone are listed. In each image 5 μl of a yeast culture of strain PJ69-4a with an optical density at 600 nm of 0.1 and transformed with the corresponding plasmids encoding the fusions proteins was spotted, incubated overnight, overlaid with X-Gal, and incubated for 16 to 24 h. Blue color indicates interaction. The quantitative determination of the β-galactosidase activity is given in arbitrary units. Error is the standard error of the mean. AD, activation domain; BD, binding domain.