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. 2006 Sep 1;5(10):1635–1647. doi: 10.1128/EC.00210-06

TABLE 2.

Oligonucleotides used in this study

Name Sequence
AgBOI1/2-S1 AGGATGAGTTTTTCGTTCAAGAAGGCGCTGAAGCTGACCGGGATGGCTAGGGATAACAGGGTAAT
AgBOI1/2-S2 TCTTTCGTGTTTATAATACAGACTTTTCAATAGCACTTGAAATCAAGGCATGCAAGCTTAGATCT
AgBOI1/2-N1 TCTTTCGTGTTTATAATACAGACTTTTCAATAGCACTTGAAATCAGGGGCAGGGCATGCTCATGTAG
AgBOI1/2-N1 TCTTTCGTGTTTATAATACAGACTTTTCAATAGCACTTGAAATCAGGGGCAGGGCATGCTCATGTAG
AgBOI1/2-G1 GAGGACTCTTGAAGGCACGG
AgBOI1/2-G4 AATTGACTCCTAACGAAGCTCC
AgBOI1/2-I AACCAGGCCGATTCTGCGG
V2-NAT1 GTGGTGAAGGACCCATCCAG
V3-NAT1 ACATGAGCATGCCCTGCCCC
AgRHO3-N1 CTGGCATCAGAGGAAGCTCCCACCACCAAGCTCTACAAACACAAGATGGGTACCACTCTTGACGAC
AgRHO3-N2 ATATTAGTATAGTCTAAAGTTGCAGGCAGTGGGTATTAAAGTTCAGGGGCAGGGCATGCTCATGTAG
AgRHO3-G1 CACGCGCAGCACGCGAGCTG
AgRHO3-G4 CGCCGTTCCGCCTCGGCCGG
AgRHO3-I CTCCGAGGATGACGATCTTG
AgBOI1/2-GFP1 TTATTTATTAATCTGGCAGGACTGCTCTTTCGTGTTTATAATACAAGGGACCTGGCACGGAGC
AgBOI1/2-GFP2 AACGAGTACTATGGCCTAGATCCGAAATATATGGGTGAGAAGATTGGTGCAGGCGCTGGAGCTG
AgCYK1-N1 GTAACGTATCGCTAGTTCAACCTACCAGCAGTGCATCGGGAGCTACTAGGATGGGTACCACTCTTGACGAC
AgCYK1-N2 CGTATTAATTATTATTGTTGTCCTTATCCATCGTTACGTAAGTATAATTAGGGGCAGGGCATGCTCATGTAG
AgCYK-G1 TAGAGACCACGGCATTTG
AgCYK-G4 GGCTGCTTTCTCCTATTG
5SmaI-AgBOI1/2 TCCCCCGGGTATGAGTTTTTCGTTCAAGAAGG
3BamHI-AgBOI1/2 CGGGATCCTCAAATCTTCTCACCCATATATTTCG
5EcoRI-AgRHO3 GATCGAATTCATGCCTCTGTGTGGGTCGAG
3BamHIAgRHO3 CGACGGATCCTAACTGCTGCTTTCGGCCTCGG
AgRHO3_G24Vfor GTGGCTTGCGGGAAGACGTCGCTG
AgRHO3_(G24V)rev GTCTCCGAGGATGACGATCTTG
AgRHO3_T29Nfor AACTCGCTGTTGAACGTGTTCAC
AgRHO3_(T29N)rev CTTCCCGCAAGCACCGTCTCC
AgRHO3_Q71Lfor CTGCACATCACGCTGAGCCTGTG
AgRHO3_(Q71L)rev GTTGTCCACGAAGATGTCATGG
AgBOI1/2ΔCfor GCGAATGGCAATGCTAACGAGTAC
AgBOI1/2ΔCrev CTTACTCATCCAACCCGAGCACGC
AgBOI1/2ΔNfor CGGAACGTTACAGTAAATGACGCTG
AgBOI1/2ΔNrev ACTGTCGTCGCCCTGCTCCATCCCG