TABLE 2.
Oligonucleotides used in this study
| Name | Sequence |
|---|---|
| AgBOI1/2-S1 | AGGATGAGTTTTTCGTTCAAGAAGGCGCTGAAGCTGACCGGGATGGCTAGGGATAACAGGGTAAT |
| AgBOI1/2-S2 | TCTTTCGTGTTTATAATACAGACTTTTCAATAGCACTTGAAATCAAGGCATGCAAGCTTAGATCT |
| AgBOI1/2-N1 | TCTTTCGTGTTTATAATACAGACTTTTCAATAGCACTTGAAATCAGGGGCAGGGCATGCTCATGTAG |
| AgBOI1/2-N1 | TCTTTCGTGTTTATAATACAGACTTTTCAATAGCACTTGAAATCAGGGGCAGGGCATGCTCATGTAG |
| AgBOI1/2-G1 | GAGGACTCTTGAAGGCACGG |
| AgBOI1/2-G4 | AATTGACTCCTAACGAAGCTCC |
| AgBOI1/2-I | AACCAGGCCGATTCTGCGG |
| V2-NAT1 | GTGGTGAAGGACCCATCCAG |
| V3-NAT1 | ACATGAGCATGCCCTGCCCC |
| AgRHO3-N1 | CTGGCATCAGAGGAAGCTCCCACCACCAAGCTCTACAAACACAAGATGGGTACCACTCTTGACGAC |
| AgRHO3-N2 | ATATTAGTATAGTCTAAAGTTGCAGGCAGTGGGTATTAAAGTTCAGGGGCAGGGCATGCTCATGTAG |
| AgRHO3-G1 | CACGCGCAGCACGCGAGCTG |
| AgRHO3-G4 | CGCCGTTCCGCCTCGGCCGG |
| AgRHO3-I | CTCCGAGGATGACGATCTTG |
| AgBOI1/2-GFP1 | TTATTTATTAATCTGGCAGGACTGCTCTTTCGTGTTTATAATACAAGGGACCTGGCACGGAGC |
| AgBOI1/2-GFP2 | AACGAGTACTATGGCCTAGATCCGAAATATATGGGTGAGAAGATTGGTGCAGGCGCTGGAGCTG |
| AgCYK1-N1 | GTAACGTATCGCTAGTTCAACCTACCAGCAGTGCATCGGGAGCTACTAGGATGGGTACCACTCTTGACGAC |
| AgCYK1-N2 | CGTATTAATTATTATTGTTGTCCTTATCCATCGTTACGTAAGTATAATTAGGGGCAGGGCATGCTCATGTAG |
| AgCYK-G1 | TAGAGACCACGGCATTTG |
| AgCYK-G4 | GGCTGCTTTCTCCTATTG |
| 5SmaI-AgBOI1/2 | TCCCCCGGGTATGAGTTTTTCGTTCAAGAAGG |
| 3BamHI-AgBOI1/2 | CGGGATCCTCAAATCTTCTCACCCATATATTTCG |
| 5EcoRI-AgRHO3 | GATCGAATTCATGCCTCTGTGTGGGTCGAG |
| 3BamHIAgRHO3 | CGACGGATCCTAACTGCTGCTTTCGGCCTCGG |
| AgRHO3_G24Vfor | GTGGCTTGCGGGAAGACGTCGCTG |
| AgRHO3_(G24V)rev | GTCTCCGAGGATGACGATCTTG |
| AgRHO3_T29Nfor | AACTCGCTGTTGAACGTGTTCAC |
| AgRHO3_(T29N)rev | CTTCCCGCAAGCACCGTCTCC |
| AgRHO3_Q71Lfor | CTGCACATCACGCTGAGCCTGTG |
| AgRHO3_(Q71L)rev | GTTGTCCACGAAGATGTCATGG |
| AgBOI1/2ΔCfor | GCGAATGGCAATGCTAACGAGTAC |
| AgBOI1/2ΔCrev | CTTACTCATCCAACCCGAGCACGC |
| AgBOI1/2ΔNfor | CGGAACGTTACAGTAAATGACGCTG |
| AgBOI1/2ΔNrev | ACTGTCGTCGCCCTGCTCCATCCCG |