FIG. 1.

Phylogenetic analysis of the L. major ABC proteins. Nucleotide-binding domains were aligned by using CLUSTAL W. The resulting multiple alignment was subjected to phylogenetic analysis by using the neighbor-joining algorithm of the MEGA software. The reliabilities of each branch point were assessed by the analysis of 1,000 bootstrap replicates. A human representative of each mammalian subfamily was incorporated in the analysis to define each subfamily. The subfamily groups are shown as bars on the right of the tree, and I and II represent the N- and C-terminal NBDs of full-length proteins, respectively. lm, Leishmania major; hs, Homo sapiens.