FIG. 5.

ABC gene expression in life stages of L. infantum. (A) Scatter plot of hybridization intensities representing the average of four independent experiments between L. infantum amastigote and promastigote parasites. The external lines indicate twofold differences, and genes whose expression differs significantly are indicated. The expression of genes represented by dots within the two external lines is considered similar in the two tested conditions, unless indicated otherwise. (B) Real-time PCR fluorescence curves representing duplicates of the ABCA3 gene amplification in promastigotes (dashed and full lines) and amastigotes (dotted and broken lines). The amplification curves of the GAPDH gene used for normalization are shown in the inset. (C) Validation of microarrays data by real-time RT-PCR. The gene ABCC4 was not found to be differentially expressed by microarrays and was used as a negative control. A mean of four independent experiments is shown for microarrays, and a mean of two independent experiments is shown for real-time RT-PCR.