FIG. 5.
Effect of farnesol concentration on biofilm formation by C. albicans GDH 2346. Farnesol, at concentrations of 50 μM, 100 μM, and 1 mM, was added at different stages of biofilm development. Biofilm formation (XTT reduction) is expressed as a percentage of that of control biofilms incubated in the absence of farnesol. Results are means ± SEMs from at least two independent experiments carried out in triplicate. Mean (±SEM) control values (A492) ranged from 1.999 ± 0.399 to 2.311 ± 0.142. Farnesol was added at adhesion (□) adhesion and time zero (▤) time zero (
), 2 h of biofilm formation (▥), 4 h of biofilm formation (░⃞), and 24 h of biofilm formation (
). ▪, control biofilm (no farnesol added). P was <0.05 (*) and <0.001 (**) for treated biofilms compared with untreated controls (Student's t test).