FIG. 7.
Signaling by glucose sensors in S. cerevisiae. The snf3Δ rgt2Δ sensor-deficient S. cerevisiae mutant strain (YM6870) carrying a PHXT1-lacZ reporter plasmid (pBM3712) was transformed with constructs expressing wild-type S. cerevisiae Rgt2 (YM7311), wild-type S. cerevisiae Snf3 (YM7312), C. albicans Hgt4 glucose-binding domain fused to the Rgt2 cytoplasmic tail (Hgt4-Rgt2 tail, YM7314), or vector only (YM7309). Values were normalized to cells harboring vector only (baseline). The β-galactosidase activity is indicated in Miller units.