TABLE 2.
Genes regulated by Hgt4 (HGT4-1 versus hgt4Δ[ρ])a
| ORF no. | Fold change
|
Gene | GO annotation | |
|---|---|---|---|---|
| 30°C | 37°C | |||
| orf19.5962 | 28.6 | 65.4 | HGT4/SNF3 | High-affinity glucose transport protein; response to glucose stimulus |
| orf19.7094 | 27.9 | 53 | HGT12/SNF32 | Glucose sensor or transporter protein; response to glucose stimulus |
| orf19.2023 | 14.8 | 29.5 | HGT7/HXT3 | Hexose transporter; fructose, mannose, glucose transport |
| orf19.4384 | 13.6 | 29.2 | HXT10 | Fructose symporter; galactose, glucose, mannose transport |
| orf19.508 | 5.83 | 5.5 | QDR1 | Multidrug resistance transporter |
| orf19.5713 | 3.48 | 3.11 | NDE1 | NADH dehydrogenase; cytochrome c oxidase, ethanol fermentation |
| orf19.5730 | 3.14 | 5.28 | orf19.13152 | 3-Polyprenyl-4-hydroxybenzoate decarboxylase |
| orf19.5337 | 3.03 | 3.65 | UBC15 | E2 ubiquitin-conjugating enzyme; ubiquitination, DNA repair |
| orf19.4527 | 2.98 | 2.98 | HGT1 | Hexose transporter |
| orf19.1048 | 2.93 | 2.39 | IFD1 | Benzyl or aryl-alcohol dehydrogenase; aldehyde metabolism |
| orf19.999 | 2.73 | 5.62 | GCA1 | Glucoamylase; alpha glucosidase, cell wall biosynthesis |
| orf19.5911 | 2.71 | 4.8 | CMK1 | Ca2+/calmodulin-dependent protein kinase; signal transduction |
| orf19.3670 | 2.64 | 2.24 | GAL1 | Galactokinase; galactose metabolism, transcription regulation |
| orf19.3392 | 2.61 | 3.04 | DOG1 | 2-Deoxyglucose-6-phosphate phosphatase; glucose metabolism |
| orf19.1509 | 2.47 | 3.37 | ROD1 | Calcium and zinc resistance protein |
| orf19.2244 | 2.46 | 2.78 | orf19.9785 | Aldoketo reductase |
| orf19.2020 | 2.45 | 3.48 | HGT6/HXT6 | Hexose transporter; fructose, mannose, glucose transport |
| orf19.4773 | 2.42 | 3.97 | AOX2 | Alternative oxidase II |
| orf19.4941 | 2.38 | 2.15 | TYE7 | Transcription factor; positive regulator of glycolysis |
| orf19.6803 | 2.36 | 2.74 | HUT1 | UDP-galactose transporter, endoplasmic reticulum translocation, galactosylation |
| orf19.4318 | 2.3 | 2.37 | MIG1 | Transcriptional regulator; transcription corepression, glucose metabolism |
| orf19.542 | 2.26 | 3.32 | HXK2 | Hexokinase II, fructose metabolism |
| orf19.6420 | 2.06 | 2.19 | PGA13 | GPI anchor, similar to mucins, regulated by Tsa1, Cyr1, Nrg1, Tup1, Rlm1 |
| orf19.5816 | 2.02 | 2.14 | EBP7 | NADPH dehydrogenase |
| orf19.2990 | 2 | 2.3 | XOG1 | Glucan 1,3-β-glucosidase; cell wall biogenesis/organization, glucan metabolism |
| orf19.1237 | 1.96 | 1.95 | ARO9 | Aromatic amino acid aminotransferase II; amino acid metabolism |
| orf19.3668 | 1.92 | 4.15 | HGT2 | Hexose transporter |
| orf19.5392 | 1.91 | 4.19 | orf19.12847 | Conserved protein; iron homeostasis siderochrome-iron transport |
| orf19.6993 | 0.44 | 0.47 | GAP2 | General amino acid permease regulated by Nrg1 and Tup1 |
| orf19.5503 | 0.44 | 0.46 | Putative ORF | Hypothetical protein |
| orf19.5975 | 0.42 | 0.5 | orf19.13396 | Zinc-finger transcription factor |
| orf19.4568 | 0.4 | 0.22 | ZCF2 | Zn(2)-Cys(6) transcription factor |
| orf19.2292 | 0.37 | 0.48 | OPT4 | Oligopeptide transporter protein |
| orf19.3902 | 0.31 | 0.42 | orf19.11383 | Hypothetical protein |
| orf19.3904 | 0.3 | 0.4 | orf19.11385 | Hypothetical protein |
a
The HGT4-1 (CM36) and hgt4Δ[ρ] null (CM32) strains were grown to log phase in synthetic complete medium with glycerol at 30°C (n = 2 each strain) and 37°C (n = 2 each strain). Total RNA was prepared and used to probe 70-mer DNA oligonucleotide microarrays representing all of the ORFs annotated in the C. albicans genome assembly 19. Genes changed by >2-fold at 30 and 37°C are shown.