FIG. 5.

Cellular contents are not degraded in the DdAtg1^K36A-expressing mutant during starvation. DH1 cells expressing DdAtg1 or DdAtg1^K36A were fixed after growth or after 36 h of starvation in growth medium without amino acids. Cells were sectioned and observed by TEM. As shown in the lower right frame, mitochondria and cytoplasm are not degraded in the cells expressing DdAtg1^K36A after 36 h in amino-acid-free FM. Normal degradation during starvation is shown in the lower left panel. The large vacuoles observed in the upper frames are part of the contractile vacuole water-pumping system and are lost during starvation. Bar, 1 μm.