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Hog1 is essential for survival under arsenite stress. Serial dilutions (1:5) of the wild type and several mutants (hog1Δ, fus3Δ, kss1Δ, ste7Δ, slt2Δ, and bck1Δ) were plated on rich medium plates (YPD) in the presence of sodium arsenite, sodium chloride, and hydrogen peroxide. All of the strains used in this report were in the genetic background of the BY series, obtained from EUROSCARF. Pictures were taken after the plates were incubated for 2 to 3 days at 24°C.
